Hydrophobicity characterization and binding studies of non-aqueous medium hydrophobic macromolecules to Vitis amurensis agglutinin II.
The nonspecific macromolecular agglutination reaction between carbohydrates and proteins and carbohydrates and lectins is known to occur in hydrophobic systems. However, the nature of the interaction between Vitis amurensis agglutinin II (VAAII) and hydrophobic macromolecules, such as polystyrene latexes, dextran, dextran sulfate (DS), polyethylene glycol (PEG) of different molecular weight, and tannin was studied in aqueous and non-aqueous solution. By fluorescence quenching assay, the maximum biding constants for VAAII and PEG in aqueous solution were calculated to be 1.78 x 10(4) and 2.42 x 10(4) L/mol, respectively. The maximum binding constants for the interaction of VAAII and PEG in hexadecane, isooctane, tricaprylin, and tannin were calculated to be 3.00 x 10(3), 1.29 x 10(3), 3.41 x 10(2), and 1.20 x 10(2) L/mol, respectively. VAAII was found to have no affinity to dextran, dextran sulfate, polyethylene glycol in hexadecane, isooctane, and tricaprylin; however, the biding constants to PEG in these two solvents were calculated to be 2.96 x 10(4) and 1.79 x 10(4) L/mol. Hence, it is concluded that there was a hydrophobic interaction between VAAII and hydrophobic macromolecules such as polystyrene latexes, dextran, dextran sulfate, polyethylene glycol, and tannin in non-aqueous medium. The results were discussed with respect to the molecular motion of the macromolecules in non-aqueous medium.NASA is the latest recipient of the U.S. National Medal of Technology for technology that has helped transform humanity’s relationship with the sun.
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